DNA Inventory
Using the same techniques and equipment
that are applied in human forensics, we use genetic data to
tie noninvasively collected samples to specific individuals.
With a variety of field techniques — most commonly a
scent-baited barbed-wire enclosure — researchers obtain
systematic collections of samples at several points in time.
After genetic methods have established which individual each
sample came from, mark-recapture models are used to estimate
the number of individuals in the study population.
Our directors
have been active participants in this field from the beginning
, and WGI has demonstrated the utility of this
approach in species ranging from sturgeon to pine marten. We believe
that our quality control methods are good enough to ensure
100% accuracy of individual assignments in most studies, and
we challenge our customers to devise blind tests that can
prove us wrong.
In our most
challenging project to date, we identified 545 individual grizzly
bears from hairs collected in the Rocky Mountains of Montana
through a project led by Katherine Kendall of the USGS. Kate didn't want any
doubts to linger over her results, so she interspersed over 700 hair samples from
known bears among her 34,000 project samples, hoping to use these blind control
samples to provide an estimate of our error rate. As we were informed after handing
over the final results, no errors were made in assigning individual identity for the 700
blind control samples. This puts the risk of laboratory errors in perspective; most
projects assign individual identity to fewer than 700 samples.
In another example, Robert Mulders(1) and colleagues have been snagging hairs
from wolverines using posts that are wrapped in barbed-wire
and topped with a bait. The method is remarkably successful,
with the majority of posts collecting wolverine hair and the majority
of individuals in the population getting 'captured' each year. The
cold, dry environment of the Barren Grounds preserves the
samples, producing unusually strong genetic results. Robert
is using the genetic data that we produce to monitor abundance
and individual movements around several diamond mining developments.
(1)Norwest Territories Dept. of Resources, Wildlife and Economic
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